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First Strand cDNA Synthesis System

System for Labeling RNA or Preparing First Strand of the cDNA

  • High efficiency, full length first strand cDNA synthesis reagents
  • Oligo dT and random primers are provided for cDNA synthesis
  • Sufficient reagents are provided for 10 reactions
  • Supplied with control templates for QC purposes

DTI’s First Strand cDNA Synthesis System provides reagents for reverse transcription of cDNA from RNA/ mRNA templates. When used with DNA Amplification System, reverse transcribed first strand of the cDNA can be amplified into double stranded DNA product for PCR amplification, gene expression profiling or cDNA identification purposes. Total RNA or mRNA from a variety of sources (human, mouse, rat, plants, bacteria, fungi, yeast) can be used as a template for cDNA synthesis or labeling probes for microarray analysis.

Kit Components

  • Reverse Transcriptase – 10 µl
  • Oligo dT Primer – 10 µl
  • Random Primer – 10 µl
  • 10x Reverse Transcriptase Buffer – 50 µl
  • 10x dNTP Mix - 50 µl
  • RNase Inhibitor - 10 µl
  • DEPC Water - 500 µl
  • Control RNA - 10 µl
  • Instruction Manual

Storage Condition and Stability

Reverse Transcription System is stored at -20o C and stable for six months.

Ordering Information
Product Catalog Number Price
Reverse Transcription System 7114 $250/ 10 Reaction
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