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DNA Blunting System

  • Convert recessed 3’- and 5’- ends for cloning purposes
  • All necessary reagents are provided with the kit

DTI’s DNA Blunting System provides reagents for converting recessed 3’- and 5’- ends for cloning purposes. The reagents provide the researcher a quick, reliable and convenient system for shotgun cloning of DNA fragments or PCR products into plasmid, cosmid or lambda phage vectors.

Applications

  • Rapid cloning of DNA for gene expression purposes
  • Construction of recombinant plasmids for shot gun libraries and DNA sequencing applications

Kit Components

  • DNA Polymerase Cocktail, 50 µl
  • 10x Blunting Reaction Buffer, 100 µl
  • 10x dNTP Mix, 100 µl
  • Nuclease Free Water, 500 µl
  • Instruction Manual

Storage Condition and Stability

Kit components are stored at -20o C and stable for 3-6 month.

Ordering Information
Item Catalog Number Price
DNA Blunting System 6612 $225/ 20 Blunting Reactions
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