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Chemical Competent Cells

For cDNA library construction and cloning applications, DNA Technologies offers high efficiency chemical competent cells. The cells are designed to obtain transformation efficiencies exceeding >1-5x 108 cfu /µg supercoiled pUC19 DNA. These cells are ideal for cloning and propagation of plasmid, cosmid, or fosmid clones. The strains allows methylated genomic DNA isolated directly from mammalian or plant cells to be cloned without deletions or re-arrangements. They give high yield and high quality plasmid DNA due to the endA1 mutation. E. coli SJ T1 cells contains tonA gene that confers resistance to bacteriophages T1 and T5.

Advantages of DTI’s Electro Competent Cells:

  • High transformation efficiency – 1-5 x 108 cfu /µg supercoiled pUC19 DNA for competent cells
  • Allows blue/white screening through ß-galactosidase complementation
  • E. coli T1 cells confers resistance to bacteriophages T1 and T5
  • Available as ready-to-use aliquot either in tubes or in 96 well format

Components

  • Competent Cells
  • pUC19 DNA
  • Instruction Manual
Ordering Information
Chemical Competent Cells* Transformation Efficiency Catalog Number Size Price
Super Chemical Competent E. coli SJ Cells 1 x 108 8104-0
8104-1
10 x 60 µl
96 x 60 µl
$75
$525
Super Chemical Competent E. coli SJ T1 Cells 1 x 108 8105-0
8105-1
10 x 60 µl
96 x 60 µl
$85
$595
Ultra Chemical Competent E. coli SJ Cells 3-5 x 108 8106-0
8106-1
10 x 60 µl
96 x 60 µl
$95
$595
Ultra Chemical Competent E. coli SJ T1 Cells 3-5 x 108 8107-0
8107-1
10 x 60 µl
96 x 60 µl
$105
$695
*Available in august 2005

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